Composite
Part:BBa_K2787003:Design
Designed by: Weiyi Tang Group: iGEM18_ShanghaiTech (2018-10-09)
Constitutive promoter J23100 + orthogonal RBS TJU + GFP+ LVA tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 27
Illegal NheI site found at 50 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 722
Design Notes
NO
Source
The part was built by inserting the o-RBS and promoter before the GFP coding sequencing provided by 2018 Distribution Kit. The orthogonal RBS sequence was published by iGEM12_Tianjin.