Composite

Part:BBa_K2787003:Design

Designed by: Weiyi Tang   Group: iGEM18_ShanghaiTech   (2018-10-09)


Constitutive promoter J23100 + orthogonal RBS TJU + GFP+ LVA tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 722


Design Notes

NO


Source

The part was built by inserting the o-RBS and promoter before the GFP coding sequencing provided by 2018 Distribution Kit. The orthogonal RBS sequence was published by iGEM12_Tianjin.

References